The only NIPT agency to
conduct inspections in Japan

Made in Japan, Analized in Japan, Customized in Japan.

No transportation problems overseas

Because fedex is not used, it is avoided from aviation trouble.
In addition, the delay of the specimen is also reduced.

The only laboratory in Japan for testing
all chromosomes for microresement syndrome

The testing agency in its home country only tests the number of opposite sexes of chromosomes 13,18,21.
Although it is a state that is accepted, in our company microrexia syndrome, the opposite sex number of all chromosomes
provides all currently possible test results, including:

Because it is domestic, it is possible
to review numerical results.

It is difficult to obtain raw data with overseas inspection results.
In our company, it is possible to review the test results retroactively.
By tracking the test results, including follow-up after birth,
We will provide more accurate inspections.


Test results can be seen not only by positive
negatives but also numerically.

Numbers or Just Judgement?

The first in the world, display the test resu
lts numerically.

Positive, not only negative, how many cut-off values,
You can see how positive it was.

For example, when got (ASP) is told to be positive when testing for liver function,
The meaning is different if the number is 50,500,1000.(Normal value is usually 40 or less)
NiPT tests can also show how positive they are.

Because you can see the number of genes that were actually matched,
You can see if it was a sufficient amount of genes.


Calculated using
the latest calculation method

The Latest Calculations!!

The latest method for detecting the amount of DNA
measured is state-of-the-art.

TOKYO CLINICAL LABORATORY uses the latest inspection methods to manage the test results.
The method of detecting the amount of DNA measured is state-of-the-art, and LLR (Log Likelhood Ratio)
A combination of both anequivalent score (t-statistics) and fetal DNA fragments is the likelihood ratio.

The log-likelihood ratio (LLR) is calculated for each region, that is, for each chromosome, excluding sex chromosomes.

In addition, by reading the cfDNA from both sides (pair end sequence),
It is possible to estimate the length of each cfDNA from the human reference sequence.
CfDNA of the fetus is known to be slightly shorter than that of the mother,
By examining the distribution of short and short cfDNA,
It is now possible to more accurately inform the chromosome ratio of the fetus (Fetal fraction:FF).

By combining the data of short fragments with the data of all fragments,
It enables highly sensitive anequil detection than LLR.

Aneal score compares the variation of the lead count,
Represents how far away it is from a positive multiple.
It is calculated from the difference in the count of chromosomes of the target chromosome and reference.
In addition, monosomy and trisomy ratio also in sex staining,
There is also the detection of the metric called NCV so far,
The value of monosomy and trisomy ratio in LLR is divided into individual specimens.
The report also added a test method to detect in more detail.

The calculation of the positive suitable medium rate is calculated by taking into account not only the pregnancy age but also the number of weeks of pregnancy.
This is because the incidence of 12,18,13 trisomy decreases with each week of pregnancy.

Machine explanation video of TOKYO CLINICAL LABORATORY

※ Please pay attention to the volume of the voice.

Visit to the Inspection Office of the TOKYO CLINICAL LABORATORY

You can also visit the inspection station at the TOKYO CLINICAL LABORATORY.
A genetic expert will guide you.
Please contact us for more information as it is a complete reservation system.

Real-time PCR

A machine for confirming amniotic fluid test.

In real-time PCR, the target gene sequence molecule is amplified exponentially to determine whether the gene exists. It is possible to measure not only that, but also the amount. When microdeletions (duplications) are confirmed by NIPT, amniotic fluid samples can be used for real-time PCR to measure the amount of genes (CNV) in that region. I will.

It is also possible to measure the amount of gene and measure the mosaic condition during chromosome trisomy.
In the G band, after culturing fetal cells in amniotic fluid, the state of the chromosome is examined by Giemsa staining. By real-time PCR, it is not possible to estimate the mosaic rate by measuring the amount of genes in chromosomes with trisomy. If the normality is 2, the complete trisomy is 3, whereas for a 50% mosaic it is 2.5.

The measurement mechanism of real-time PCR is as follows.

Check using fluorescent reagents. There is a probe called a quencher in which a luminescent dye is attached to single-stranded DNA having a complementary sequence to a target gene.

The fluorescent dye is absorbed by the quencher. An enzyme called polymerase extends from the primer and DNA synthesis begins.

When the probe is decomposed by the polymerase, the fluorescent dye separates from the quencher, and the distance increases and the absorption of the quencher is limited, and fluorescence is emitted.

The presence of the gene of interest can be detected by the real-time PCR device by this fluorescence.
Used for confirming microdeletions and microduplications in all chromosomal regions. For amniotic fluid testing.